Products Description

The Bacstain series can be used for microorganism staining as indicated in Fig. 1. CFDA is used for staining of viable microorganisms. CFDA is bacterial cell wall and cell membrane permeable, and remains in the cell after being hydrolized by esterase. Other Bacstain reagents are used for nucleotide staining and are cell wall permeable, except for PI. Therefore, using one of these nucleotide staining reagents and CFDA or Bacstain-CTC Rapid Staining Kit, it is possible to stain both cells with intact membranes and cells with damaged membranes simultaneously. Since PI can stain only membrane-damaged cells, it does not stain membrane-intact cells. PI is also used for double staining coupled with CFDA.