Amine Coupling Kit
Self Assembled Monolayer Kit
- Immobilization of protein/peptides onto SAMs with terminal carboxylic acid
- Including all necessary reagents for activation, immobilization, and blocking
- Sufficient for 40 immobilizations
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Product codeA515 Amine Coupling Kit
| Unit size | -- | |
|---|---|---|
| 2 ml x 4 | Please inquire distributors about price. | |
| 2 ml x 4 | [2ml x 4] WSC NHS Activation Buffer Reaction Buffer Blocking Solution |
4 tubes 4 tubes 20 ml x 1 10 ml x 1 20 ml x 1 |
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Description
Amine and carboxylic acid coupling is one of the most common methods to immobilize a protein or a peptide through a covalent bound onto biosensor surface. Dojindo’s Amine Coupling Kit contains all the reagents and buffers necessary reagents and buffers for activation of carboxylic acid, protein immobilization, and blocking. Blocking solution included in the kit minimizes non-specific protein absorption to the surface by capping residual activated esters. Each kit is adequate for approximately 40 immobilizations.
Manual
Technical info
Example of Solvents
100 mmol/l (NHS solution)： Dissolve NHS １ tube in Activation Buffer 1 ml in the kit
100 mmol/l (WSC solution)： Dissolve WSC 1 tube in Activation Buffer 1 ml in the kit
References
Expression and assembly of active human cardiac troponin in Escherichia coli MW Lassalle - Protein expression and purification, 2012
Handling and storage condition
| 0-5°C | |
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Danger / harmful symbol mark |
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