Quin 2
Reagent for Monitoring Calcium Ion
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Product codeQ001 Quin 2
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CAS No.73630-23-6
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Chemical name8-Amino-2-[(2-amino-5-methylphenoxy)methyl]-6-methoxyquinoline-N,N,N',N'-tetraacetic acid, tetrapotassium salt
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MWC26H23K4N3O10=693.87
| Unit size | -- | |
|---|---|---|
| 50 mg | Please inquire distributors about price. | |
Product Description
Quin 2 forms a stable fluorescent complex with calcium (logKCaY=7.1), but not with magnesium (logMgY=2.7). The complex has a high quantum yield (0.14) at emission wavelength 525 nm, excitation wavelength 339 nm, and emission wavelength 492 nm. Quin 2-AM is an acetoxymethyl ester derivative of Quin 2 that is readily permeable through cell membranes. Within the cell, the ester is hydrolyzed to Quin 2.Quin 2 forms a stable fluorescent complex with calcium (logKCaY=7.1), but not with magnesium (logMgY=2.7). The complex has a high quantum yield (0.14) at emission wavelength 525 nm, excitation wavelength 339 nm, and emission wavelength 492 nm. Quin 2-AM is an acetoxymethyl ester derivative of Quin 2 that is readily permeable through cell membranes. Within the cell, the ester is hydrolyzed to Quin 2.
Calcium Chelation
References
1. R. Y. Tsien, New Calcium Indicators and Buffers with High Selectivity against Magnesium and Protons: Design, Synthesis, and Properties of Prototype Structures. Biochemistry. 1980;19:2396-2404.
2. R. Y. Tsien, et al., Calcium Homeostasis in Intact Lymphocytes: Cytoplasmic Free Calcium Monitored with a New, Intracellularly Trapped Fluorescent Indicator. J Cell Biol. 1982;94:325-334.
3. M. B. Feinstein, et al., The Cytoplasmic Concentration of Free Calcium Inplatelets Is Controlled by Stimulators of Cyclic AMP Production (PGD2, PGE1, FORSKOLIN). Biochem Biophys Res Commun. 1983;113:598-604.
4. N. Miyoshi, et al., A New Method of Determining Intracellular Free Ca2+ Concentration Using Quin2-fluorescence. Photochem Photobiol. 1991;53:415-418.
Handling and storage condition
| Appearance： | Pale yellow powder |
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| Purity (HPLC)： | ≧ 95.0 % |
| Solubility in water： | To pass test (clear, pale yellow) |
| Molar absorptivity： | ≧ 34,000 (around 240 nm) |
| Protect from light |
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