Sodium bicinchoninate
Metal Indicator
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Product codeB037 Sodium bicinchoninate
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CAS No.979-88-4
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Chemical name4,4'-Dicarboxy-2,2'-biquinoline, disodium salt
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MWC20H10N2Na2O4=388.28
| Unit size | Price | Item Code |
|---|---|---|
| 5 g | B037-10 |
Product Description
Sodium bicinchoninate is a Cu(I)- specific colorimetric reagent that is more sensitive than neocuproine. Its absorption maximum is at 562 nm (molar absorptivity: 7,900). In alkaline solution, Cu(II) is reduced by proteins to Cu(I), and then forms a purple complex with Sodium bicinchoninate. The protein detection range of this assay is 1-2,000 mg per ml.
Chemical Structure
References
1) K. Ueno and K. Kina, "Colloid Titration - A Rapid Method for the Determination of Charged Colloid", J. Chem. Education, 1985, 62, 627.
2) P. K. Smithm, R. I. Krohn, G. T. Hermanson, A. K. Mallia, F. H. Gartner, M. D. Provenzano, E. K. Fujimoto, N. M. Goeke, B. J. Olson and D. C. Klenk, "Measurement of Protein Using Bicinchoninic Acid", Anal. Biochem., 1985, 150, 76.
3) M. G. Redinbaugh and R. B. Turley, "Adaptation of the Bicinchoninic Acid Protein Assay for Use with Microtiter Plates and Sucrose Gradient Fractions", Anal. Biochem., 1986, 153, 267.
Handling and storage condition
| Appearance： | White to yellow powder |
|---|---|
| Solubility in water： | To pass test (clear, colorless or pale yellow) |
| Molar absorptivity： | ≧ 15,500 (around 332 nm) |
| Sulfated ash： | 30.0 - 40.0 % |
| Blank (protein assay)： | To pass test |
| NMR spectrum： | Authentic |
| Ambient temperature |
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