Biotin-SAM Formation Reagent
![Biotin-SAM Formation Reagent](./img/1560584580_1.jpg)
Self Assembled Monolayer Reagent
- Simple preparation of Biotin-SAM on gold surface
- Efficient Immobilization of Avidins
- Suppress unspecific binding with unknown proteins
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Product codeB564 Biotin-SAM Formation Reagent
Unit size | Price | Item Code |
---|---|---|
1 umol x 3 | B564-10 |
Product Description
There are three ways to immobilize a protein on a Surface Plasmon Resonance (SPR) or Quartz Crystal Microbalance (QCM) biosensor consisting of Self-Assembled Monolayer (SAM); 1) Formation of covalent bond of amine functions on proteins and activated carboxy-SAM; 2) immobilize proteins by His-Tag through Ni-NTA-terminated SAM; 3) Immobilization of proteins through Biotin and Avidin interaction. Because the Biotin-Avidin formation is known as a facile reaction, it is widely utilized for immobilization of protein (Fig 1).
Fig.1 A biosensor construction by Biotin-SAM Formation Reagent
Fig. 2 Comparison of the amount of bound streptavidin, unspecific binding of FBS, bound biotinylated BSA between Biotin-SAM Formation Reagent and Company A.
Manual
Handling and storage condition
0-5°C |