09 Cross-Linking Reagents

Isothiocyanobenzyl-NTA

Isothiocyanobenzyl-NTA

Chelate Labeling Reagent

  • Product code
    I279  Isothiocyanobenzyl-NTA
  • Chemical name
    N-[5-(4-IsothiOcyanatobenzyl)amido-1-carboxypentyl]iminodiacetic acid
  • MW
    C19H23N3O7S=437.47
Unit size Price Item Code
10 mg I279-10

Product Description

Isothiocyanobenzyl-NTA is utilized to modify the surfaces on which amine groups are attached. Through the NTA moiety attached on the surface, genetically expressed protein, bearing a hexahistidine extension at its terminus, can be immobilized via Ni (II) (His-Tag method). Using this technique, Dr. Noji and co-workers were able to directly abserve the rotation of F1-ATPase with a fluorescence microscope.

Chemical Structure

Manual

Technical info

Handling and storage condition

Specification
Appearance: White to slightly yellow powder
Purity (HPLC): ≧ 90.0 %
IR spectrum: Authentic
NMR spectrum: Authentic
Handling and storage condition
-20°C
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Product Classification

Product Classification

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