Sulfo-GMBS

09 Cross-Linking Reagents

Sulfo-GMBS

Sulfo-GMBS

Cross-Linker

  • Product code
    S025　 Sulfo-GMBS
  • CAS No.
    185332-92-7
  • Chemical name
    N-(4-Maleimidobutyryloxy)sulfosuccinimide, sodium salt
  • MW
    C12H11N2NaO9S=382.28
Unit size Price Item Code
50 mg S025-10

Description

Cross-linking Reaction

 

Product Description
Hetero-bifunctional cross-linking reagents have activated esters and maleimide reactive groups. These functional groups react with amines and sulfhydryl groups of proteins, respectively. Enzyme-labeled haptens are prepared using hetero-bifunctional cross-linking reagents such as EMCS or GMBS. The cross-linking reaction requires neutral pH and mild temperature because it is necessary to maintain enzyme activity and antibody titers in the cross-linking reaction. Hetero-bifunctional cross-linking reagents with 3, 5, 7, or 10 linear carbon chains are available. These linear aliphatic chains act as spacers between the two reactive sites and their water-soluble reagents. They are more stable than the aromatic cross-linking reagents such as succinimidyl-4-N-maleimidobenzoate in a wider pH range.

Conjugation of Macromolecules with Hetero-Bifunctional Cross-Linking Reagent

 

References

Open References

1) J. V. Staros, "N-Hydroxysulfosuccinimide Active Esters:Bis(N-hydroxysulfosuccinimide) Esters of Two Dicarboxylic acids Are Hydrophilic, Membrane-impermeant, Protein Cross-linkers", Biochemistry, 1982, 21, 3950.
2) P. S. R. Anjaneyulu and James V. Staros, "Reactions of N-Hydroxysulfosuccinimide Active Esters", Int. J. Peptide Protein Res., 1987, 30, 117. 
3) Y. Fukami, K. Sato, K. Ikeda, K. Kamisango, K. Koizumi and T. Matsuno, "Evidence for Autoinhibitory Regulation of the c-src Gene Product a Possible Interaction Between the Src Homology 2 Domain and Autophosphorylation Site", J. Biol. Chem., 1993, 268, 1132

Handling and storage condition

Specification
Appearance： White to slightly yellowish pink powder
Purity (HPLC)： ≧ 90.0 %
Solubility in water： To pass test (clear, colorless or slightly yellow)
IR spectrum： Authentic
NMR spectrum： Authentic
Handling and storage condition
0-5°C
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Product Classification

Product Classification

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