Increase Your Cytotoxicity Data Reliability with Cytotoxicity LDH Assay Kit-WST

Increase Your Cytotoxicity Data Reliability with Cytotoxicity LDH Assay Kit-WST

Enzyme-based cytotoxicity assay using MTT and WST dyes rely on a reductive coloring reagent and dehydrogenase in a viable cell to determine the cell viability. Therefore, even if the result shows cytotoxicity, it is difficult to say whether the dehydrogenase activity of the cells is lowered or it is due to cell membrane damage. In recent years, as another indicator, LDH is measured as supporting data for cytotoxicity. Learn more about cell proliferation/cytotoxicity assay here.

Here is the list of some articles which utilize multiple methods to increase data reliability: 

 

Cell Type	Treatment	Methods	Title	Reference
C3H10T1/2	Pirfenidone	CCK-8 LDH	XL413, a cell division cycle 7 kinase inhibitor enhanced the anti-fibrotic effect of pirfenidone on TGF-ß1-stimulated C3H10T1/2 cells via Smad2/4	S. Jin, et. al, Exp Cell Res., 2015, 339(2), 289-99
human L02 hepatocyte (human liver cell)	S17092 and Vehicle (dimethylsulfoxide, DMSO)	CCK-8 LDH	Prolyl Oligopeptidase Inhibition Attenuates Steatosis in the L02 Human Liver Cell Line	Zhou D, et al., PLoS ONE, 2016, 11(10)
RAW264.7	L FAC and IFN-gamma	CCK-8 LDH	Iron Reduces M1 Macrophage Polarization in RAW264.7 Macrophages Associated with Inhibition of STAT1	Zhen-Shun Gan, et. al, Mediators of Inflammation, 2017
mouse embryonic fibroblast (MEF)	Methylmercury	CCK-8 LDH	Atg5-dependent autophagy plays a protective role against methylmercury-induced cytotoxicity	Takanezawa Yasukazu, et. al, Toxicity Letters, 2016, 262, 135-141
HepG2 NHDF	Silver Nanoparticles	MTT LDH	A.Patlolla, S. Hussain, P. Tchounwou, Sixth International Symposium on Recent Advances in Environmental Health Research	A.Patlolla, et. al., Sixth International Symposium on Recent Advances in Environmental Health Research
HTC HepG2	Cadmium Chloride	MTT LDH	In vitro cytotoxicity assays: Comparison of LDH, neutral red, MTT and protein assay in hepatoma cell lines following exposure to cadmium chloride	G. Fotakis, et. al, Toxicology Letters, 2006, 160, 171-177
human gingival fibroblasts	Resin Composite Monomers	MTT LDH	Resin composite monomers alter MTT and LDH activity od human gingival fibroblasts in vitro	Y. Issac, D.C Watts, et. Al., Dental Material., 2004, 20 (1), 12-20