Functional Foods Evaluation

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Antioxidant Capacity Assay Kit

SOD Assay Kit

Superoxide dismutase(SOD) is an antioxidant enzyme that exists in the body and functions to eliminate the reactive oxygen species(ROS) superoxide(O2-).
This kit can easily measure SOD-Like activity using a 96-well microplate.

Simple Protocol

Applicable with a 96 well plate. Only 1 hour to complete the entire process.

Application Data

SOD activity of Goishicha(Green Tea) in the manufacturing process.

SOD activity was greatly increased from aerobic and anaerobic fermentation from microorganisms involved in the tea manufacturing process.

Shimura, “Changes in Catechin Content and Superoxide Anion Scavenging activity of Goishi Tea During Manufacturing”, Nippon Shokuhin Kagaku Kogaku Kaishi, 55(12),640.


DPPH Antioxidant Assay Kit

In recent years, it has been suggested that a decrease in the body’s antioxidant capacity is involved in the development of various diseases and health problems, and expectations for foods with antioxidant activity(antioxidant foods) are increasing. Kouchi University’s Shimamura et al. have developed DPPH to evaluate antioxidant activity with little difference between measurement facilities. A measurement method using (2,2-Diphenyl-1-picrylhydrazyl) has been reported1). This is a microplate evaluation based on the measurement method of Shimamura et al. by manualizing the DPPH measurement method and making a kit of stable and constant quality, we are able to reduce the data variability and complexity of reagent preparation, which has had issues until now.
This product was commercialized under the guidance of Prof. Tomoko Shimamura, Department of Agricultural Chemistry, Faculty of Agriculture, Forestry and Marine Science, Kochi University.

T. Shimamura et al., Anal. Sci., 2014, 30, 717-721.

Significant reduction in reagent preparation time
High Reproducibility

Until now, it has been difficult to reproduce data between facilities and/or measurement days. This kit has the reproducibility of DPPH measurement possible by the quality of reagents used and convenient measurement and analysis technique.

Stable Quality of Reagent
The quality of DPPH greatly improves the measurement results by using a highly stable quality of DPPH.

Optimized Measurement Condition
The pH and solvent are factors measurement errors in antioxidant capacity.
This kit allows assay without influential factors.
Analysis by Comparison using Trolox Standard
Improve daily data variance and data variance in facility by the Trolox-based evaluation(TEAC) method.
Comparison with a Conventional Method

When measuring antioxidant activity with DPPH, the pH and solvent concentration in the solution affect the measurement.
This kit uses protocols and analysis methods to minimize these effects.

Effect of pH

The assay buffer provided with the product allows measurement at a certain pH.

Effect of sample solvent

The quality of DPPH greatly improves the measurement results by using a highly stable quality of DPPH.
Simple Protocol

After preparing the reagent, simply add the reagent and sample to the 96 well microplates and react for 30 minutes.

SOD or SOD-like Activity Detection (Relevant to antioxidant capacity)

Features

  • WST-1 based SOD inhibition assay
  • Colorimetric microplate measurement
  • Measures 100% inhibition by SOD
  • pH-independent IC50 determination
  • Low background noise measurement

Antioxidant capacity Detection

Features

  • Reproducible detection of antioxidant capacity assay using a DPPH-method
  • Significant reduction in reagent preparation time
  • High reproducibility


ACE Inhibition Activity Assay Kit

ACE Kit-WST

Studies have shown that the enzyme “ACE(Angiotensin-converting enzyme)” is greatly involved in hypertension disease. Foods that inhibit the action of ACE are considered functional foods that help suppress increases in blood pressure. Functional label food and food for specified health use have gained a lot of attention due to these studies. This kit can easily evaluate whether a food sample has the ability to lowering blood pressure.

Features
Easy for Beginners to Use
Available in a variety of unit sizes (50 tests or 100 tests) Protocol available for determining the presence or absence of ACE inhibition activity.
All in One
Contains all the reagents required for the experiment1).
Screening for Multiple Samples
Assays can typically be completed in 2 hours or less.

※1) Required equipment and materials: Plate reader(450 nm filter), micropipette, multichannel pipette, incubator and syringe.

Protocol

Add the sample and reagent to the plate, incubate(60 min, 10 min), and measure the absorbance.

Reliable Cytotoxicity Data Reliability With Cytotoxicity LDH Assay Kit-WST

Evaluation of crop processing

Asao et al. of Nara Prefectural Agricultural Research Center measured ACE inhibition activity(IC50) for each ripening process in the sample (Angerica acutiloba Kitagawa). The ACE increased dramatically in each preparation process.

Angiotensin-Converting Enzyme (ACE) Inhibition in foods (Relevant to blood pressure)

Features

  • Colorimetric Microplate Assay Possible
  • Possible to Test Multiple Samples at One Time
  • Simple Protocol
  • No Harmful Organic Solvent Required
  • High Reproducibility

COVID-19 Announcement

Due to rising concerns regarding the COVID-19 (Coronavirus 2019) outbreak, Dojindo Molecular Technologies, Inc will be taking the necessary steps to prioritize public health and safety.

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