Glucose Uptake Assay Kit – Blue, -Green, -Red
Why has glucose uptake capacity been the focus of so much attention?
Typically, diabetes is caused by decreased insulin secretion and responsiveness. When insulin binds to insulin receptors on the cell membranes of muscle cells or adipocytes, glucose transporter 4 (GLUT4) translocates from vesicles to the cell membrane via signal transduction, which enhances the cellular uptake of glucose. In the case of diabetes, cellular uptake of glucose is inhibited. Therefore, glucose uptake capacity is one of the important indicators in the field of diabetes research.
In order to maintain active cell growth, cancer cells rapidly take in and metabolize large amounts of nutrients such as glucose to synthesize proteins and produce energy such as ATP. In addition, even under unfavorable conditions for cells (hypoxia and low nutrition), cancer cells can survive by altering their metabolic system. Therefore, in recent years, research to elucidate the metabolic system of cancer cells has been actively pursued.
Anticancer Drug/Cancer Immunity
Since cancer cells mainly use the glycolysis to produce ATP, the development of anticancer drugs that inhibit glucose transporters (GLUTs), the target proteins of the glycolysis, is underway in the field of drug discovery (right figure). In addition, in the field of cancer immunology, it has been reported that low glucose status in the tumor microenvironment causes a decrease in immune cell function as a result of enhanced glucose uptake by aerobic glycolysis in cancer cells.
Alternative to radioisotope (RI)-labeled glucose
One common method for evaluating the glucose uptake ability of cells uses radioisotope (RI)-labeled glucose. Although this method has been used for many years, it requires special handling facilities and disposal of radioactive materials. One alternative, the enzyme cycling method using 2-deoxy-D-glucose, enables colorimetric and fluorometric plate assays. However, it cannot be applied to cell imaging and flow cytometry.
Thus, we have developed novel fluorescent probes, Glucose Uptake Probe-Blue (UP01), -Green (UP02), and –Red (UP03) to overcome these limitations. These probes emit strong fluorescence, allowing easy detection of cellular glucose uptake by microplate reader and flow cytometry.
We have several colors of assay kits measuring glucose uptake capacity. Please select the kit that best suits your experiment.
|Glucose Uptake Probe-Blue||Glucose Uptake Probe-Green||Glucose Uptake Probe-Red|
|Size||1 set||1 set||1 set|
|Ex/Em||386/474 nm||507/518 nm||560/572 nm|
The Glucose Uptake Probe included in this kit is a fluorescently labeled glucose analogue similar to the conventional product 2-NBDG. These glucose analogues are taken up into cells via glucose transporters, and the glucose uptake capacity of cells can be measured by fluorescence measurement methods such as fluorescence microscopy.
∼ Features ∼
– Highly sensitive and simple measurement of glucose uptake capacity
– Reduced dye leakage after staining
– 3 colors available (blue, green, and red)
– Compatible with plate reader (UP02, UP03) and flow cytometry
– No need to dispose of radioactive waste
The use of a high fluorescence intensity dye enables higher sensitivity measurements in a shorter time than the conventional method (2-NBDG).
Experimental example: Glucose uptake enhancement by insulin
Using these kits, we were able to measure enhanced insulin-induced glucose uptake in adipocytes with high sensitivity.
Experimental example: Co-staining with Lipid Droplet Probe
Using these kits, we were able to measure glucose uptake and detect lipid droplet via simultaneous staining with a lipid droplet probe.
|Lipid droplet imaging||LD01||Lipi-Blue||10 nmol|
|LD04||Lipi-Deep Red||10 nmol|
Experimental example: Inhibition of glucose uptake by Cytochalasin B
Using these kits, we were able to detect the inhibition of glucose uptake by the glucose transporter inhibitor Cytochalasin B in HepG2 cells with high sensitivity.
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