Glucose Uptake Assay Kit – Blue, -Green, -Red

Why has glucose uptake capacity been the focus of so much attention?

Diabetes Research

Typically, diabetes is caused by decreased insulin secretion and responsiveness. When insulin binds to insulin receptors on the cell membranes of muscle cells or adipocytes, glucose transporter 4 (GLUT4) translocates from vesicles to the cell membrane via signal transduction, which enhances the cellular uptake of glucose. In the case of diabetes, cellular uptake of glucose is inhibited. Therefore, glucose uptake capacity is one of the important indicators in the field of diabetes research.

Cancer Research

In order to maintain active cell growth, cancer cells rapidly take in and metabolize large amounts of nutrients such as glucose to synthesize proteins and produce energy such as ATP. In addition, even under unfavorable conditions for cells (hypoxia and low nutrition), cancer cells can survive by altering their metabolic system. Therefore, in recent years, research to elucidate the metabolic system of cancer cells has been actively pursued.

Anticancer Drug/Cancer Immunity

Since cancer cells mainly use the glycolysis to produce ATP, the development of anticancer drugs that inhibit glucose transporters (GLUTs), the target proteins of the glycolysis, is underway in the field of drug discovery (right figure). In addition, in the field of cancer immunology, it has been reported that low glucose status in the tumor microenvironment causes a decrease in immune cell function as a result of enhanced glucose uptake by aerobic glycolysis in cancer cells.

Alternative to radioisotope (RI)-labeled glucose

One common method for evaluating the glucose uptake ability of cells uses radioisotope (RI)-labeled glucose. Although this method has been used for many years, it requires special handling facilities and disposal of radioactive materials. One alternative, the enzyme cycling method using 2-deoxy-D-glucose, enables colorimetric and fluorometric plate assays. However, it cannot be applied to cell imaging and flow cytometry.

Thus, we have developed novel fluorescent probes, Glucose Uptake Probe-Blue (UP01), -Green (UP02), and –Red (UP03) to overcome these limitations. These probes emit strong fluorescence, allowing easy detection of cellular glucose uptake by microplate reader and flow cytometry.

We have several colors of assay kits measuring glucose uptake capacity. Please select the kit that best suits your experiment.

Glucose Uptake Probe-BlueGlucose Uptake Probe-GreenGlucose Uptake Probe-Red
Product CodeUP01UP02UP03
Size1 set1 set1 set
Ex/Em386/474 nm

507/518 nm

560/572 nm

Fluorescence microscope
Flow cytometry
Microplate reader

Principle

The Glucose Uptake Probe included in this kit is a fluorescently labeled glucose analogue similar to the conventional product 2-NBDG. These glucose analogues are taken up into cells via glucose transporters, and the glucose uptake capacity of cells can be measured by fluorescence measurement methods such as fluorescence microscopy.

∼ Features ∼
– Highly sensitive and simple measurement of glucose uptake capacity
– Reduced dye leakage after staining
– 3 colors available (blue, green, and red)
– Compatible with plate reader (UP02, UP03) and flow cytometry
– No need to dispose of radioactive waste

The use of a high fluorescence intensity dye enables higher sensitivity measurements in a shorter time than the conventional method (2-NBDG).

Experimental example: Glucose uptake enhancement by insulin

Using these kits, we were able to measure enhanced insulin-induced glucose uptake in adipocytes with high sensitivity.

Please refer to the link below for details regarding Insulin treatment.
Insulin, diabetes research

Experimental example: Co-staining with Lipid Droplet Probe

Using these kits, we were able to measure  glucose uptake and detect lipid droplet via simultaneous staining with a lipid droplet probe.

Red: Lipid droplet (Lipi-Red)
Blue: Glucose Uptake Probe-Blue
Blue: Lipid droplet (Lipi-Blue)
Green: Glucose Uptake Probe-Green
Green: Lipid droplet (Lipi-Green)
Red: Glucose Uptake Probe-Red
FunctionProduct CodeProductSize
Lipid droplet imagingLD01Lipi-Blue10 nmol
LD02Lipi-Green10 nmol
LD03Lipi-Red100 nmol
LD04Lipi-Deep Red10 nmol

Experimental example: Inhibition of glucose uptake by Cytochalasin B

Using these kits, we were able to detect the inhibition of glucose uptake by the glucose transporter inhibitor Cytochalasin B in HepG2 cells with high sensitivity.

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FunctionProduct CodeProductSize
Glucose uptake detectionUP01Glucose Uptake Assay Kit-Blue1 set
Glucose uptake detectionUP02Glucose Uptake Assay Kit-Green1 set
Glucose uptake detectionUP03Glucose Uptake Assay Kit-Red1 set

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