Mitochondria Research

The multi-functional organelle is not only involved in energy production in a cell, but other additional cellular functions. The active cycle of mitochondrial fusion and division induces morphological changes, which is called mitochondrial dynamics. Abnormalities in morphological control of mitochondria are associated with neurodegenerative diseases, metabolic disorders, aging, and so on. Therefore, the demand for long-term observation of mitochondrial dynamics has recently been increasing.



 

MitophagyLipophilic peroxide / Singlet oxygen / Superoxide Membrane potential / Iron ion (Fe2+) / Calcium ion (Ca2+) Mitochondria staining
Mitophagy
ReagentMtphagy DyeKeima-Red
PrincipleMtphagy Dye (included in Mitophagy Detection Kit) is a pH-sensitive fluorescent probe that accumulates in mitochondria and emits red fluorescence due to acidic conditions in a lysosome.A pH-sentitive ratiometric fluorescent protein. The excitation spectrum changes accoring to pH. This protein shows high fluorescence ratio (Ex. 550 nm/440 nm) values in a lysozome.
Fixed cell staining
Live-cell stainingYesYes
Fixation after live-cell staining
Staining time> 30 min
Ex / Em530 / 700440, 550 / 620
Product codeMD01MT02
Lipophilic peroxideSinglet oxygenSuperoxide
Reagent MitoPeDPP Si-DMA MitoSOX
PrincipleA cell-permeant fluorescent probe that accumulates in mitochondria and specifically reacts with lipophilic peroxides in mitochondria to emit fluorescence.A cell-permeant fluorescent probe that accumulates in mitochondria and specifically reacts with singlet oxigen generated in mitochondria to emit red fluorescence.A cell-permeant fluorescent probe that accumulates in mitochondria and reacts with superoxide generated in mitochondria to emit red fluorescence.
Fixed cell staining
Live-cell stainingYesYesYes
Fixation after live-cell staining
Staining time> 15 min> 45 min> 10 min
Ex / Em452 / 470644 / 670510 / 590
Product codeM466MT05
Membrane potential Iron ion (Fe2+) Calcium ion (Ca2+)
Reagent JC-1MT-1 TMRM, TMRE Mito-FerroGreen Rhod2-AM
PrincipleA cell-permeant ratiometric fluorescent dye that accumulates in intact mitochondria due to the membrane potential. The excitation spectrum changes according to the mitochondria membrane potential.Cell-permeant fluorescent dyes that accumulate in intact mitochondria due to the membrane potential. MT-1 is extremely photostable and more sensitive than JC-1 and can provide equivalent detection sensitivity to TMRE.Cell-permeant fluorescent dyes that accumulate in intact mitochondria due to the membrane potential. Diffusion of the probes occurs in a damaged mitochondria that has the decreased membrane potential.A cell-permeant fluorescent probe that accumulates in mitochondria and specifically reacts with ferrous ion in mitochondria to emit green fluorescence.A cell-permeant fluorescent probe that accumulates in mitochondria and specifically reacts with calcium ion in mitochondria to emit red fluorescence.
Fixed cell staining
Live-cell stainingYesYesYesYesYes
Fixation after live-cell stainingYes
Staining time30-60 min30 min30-60 min30 min30-60 min
Ex / EmMonomer: 514 / 529
J-aggregation: 585/590
530-560 / 570-640550 / 575505 / 535553 / 576
Product codeMT09MT13M489R002
Mitochondria staining
ReagentMitoBright LT seriesMitoBright IM RedMitoTracker seriesMitoRedRhodamine 123
PrincipleCell-permeant fluorescent dyes that accumulate in intact mitochondria due to the membrane potential.Cell-permeant fluorescent dyes that accumulate in intact mitochondria due to the membrane potential and covalently binds to proteins and other biomolecules.Cell-permeant fluorescent dyes that accumulate in intact mitochondria due to the membrane potential.Cell-permeant fluorescent dye that accumulates in intact mitochondria due to the membrane potential.Cell-permeant fluorescent dye that accumulates in intact mitochondria due to the membrane potential.
Fixed cell staining
Live-cell stainingYesYesYesYesYes
Fixation after live-cell stainingYesYes
Staining time30 min30 min15-45 min> 15 min> 15 min
Ex / Em493 / 508, 547 / 563, 643 / 663548 / 566490 / 516 ~
644 / 665
560 / 580507 / 529
Product codeMT10,MT11,MT12MT15R237R233
Mitochondria Research
ApplicationProducts
Mitophagy DetectionMitophagy Detection Kit
Mitochondrial Phospholipid Peroxidase DetectionMitoPeDPP
Mitochondrial Ferrous Ion DetectionMito-FerroGreen
Mitochondrial Singlet Oxygen DetectionSi-DMA for Mitochondrial Singlet Oxygen Imaging
Mitochondrial Membrane PotentialJC-1 MitoMP Detection Kit
MT-1 MitoMP Detection Kit
Mitochondria StainingMitoBright LT Green
MitoBright LT Red
MitoBright LT Deep Red
MitoBright IM Red for Immunostaining
-Cellstain- MitoRed
-Cellstain- Rh123
Mitochondrial Superoxide DetectionmtSOX Deep Red – Mitochondrial Superoxide Detection

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