Amine and carboxylic acid coupling is one of the most common methods to immobilize a protein or a peptide through a covalent bound onto biosensor surface. Dojindo’s Amine Coupling Kit contains all the reagents and buffers necessary reagents and buffers for activation of carboxylic acid, protein immobilization, and blocking. Blocking solution included in the kit minimizes non-specific protein absorption to the surface by capping residual activated esters. Each kit is adequate for approximately 40 immobilizations.
Fig.1 A biosensor prepared by Amine Coupling Kit