-Bacstain- AO Solution


– Fluorescence detection
– Ready-to-use solution

Chemical Name: 3,6-Bis(dimethylamino)acridine hydrochloride, solution
CAS: 65-62-2-AO

Contents of the Kits: AO solution 75 μl x 4 vials

Storage Condition:  -20ºC, protect from light
Shipping Condition: ambient temperature

Required Equipment and Materials
10 μl, 1000 μl pipettes, incubator, Microscope (blue excitation filter and red emission filter) or flow cytometer (488 nm blue laser)


Mix and match products can be applied.
You must enter coupon code on your order to receive the discount!
・Summer 21-2 (for buying 2)
・Summer 21-3 (for buying 3 and more)
(Can not be combined with any other offer or special pricing products.)

Fig. 1 Cell staining mechanism

Staining procedure
1. Allow AO solutiona) to stand at room temperature for 30 minutes to thaw. Solution should be protected from light.
2. Resuspend the organisms with PBS(-) or saline and adjust the number of cells to 106 cells/mL(flow cytometry) or 108-109 cells/mL(microscopy).
3. Add 3 μl of AO solution into the 1 mL of microbial cell suspension and vortex gently to mix. Formaldehyde-fixation can be carried out if necessary.
4. Incubate the microbial cells at room temperature for 5 minutes.
5. Analyze the stained-cells with a flow cytometer or a microscope. The maximum wavelengths of the dye with ssDNA are 420-460 nm for excitation and 630-650 nm for emission. The maximum wavelengths of the dye with dsDNA are 500 nm for excitation and 520 nm for emission.

a) Since AO may be carcinogenic, be careful when handling and disposing.

1) J. E. Hobbie, et al., Use of Nucleopore Filters for Counting Bacteria by Fluorescence Microscopy. Appl Environ Microbiol. 1997;33:1225-1228.
2) S. F. Nishino, et al., Direct Acridine Orange Counting of Bacteria Preserved with Acidified Lugol Iodine. Appl Environ Microbiol. 1986;52:602-604.

Staining Data

Fig. 2 B. subtils stained with AO.

Related Categories
Microbial Viability Assay

Related Products

COVID-19 Announcement

Due to rising concerns regarding the COVID-19 (Coronavirus 2019) outbreak, Dojindo Molecular Technologies, Inc will be taking the necessary steps to prioritize public health and safety.

Scroll to Top