-Bacstain- Bacterial Viability Detection Kit-DAPI/PI


∼ Features ~
– Simple Procedure
– Multichannel Evaluation for Drug efficacy and Bacterial condition
– Vastly Reduce Experimental Time

Content: 1 set
Storage Condition: Store at -20 oC
Shipping Condition: Ambient Temperature

Observe Bacteria  Biofilm Assay


Mix and match products can be applied.
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・Winter 22-2 (for buying 2)
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(Can not be combined with any other offer or special pricing products.)
Detection Principle

-Bacstain- Bacterial Viability Detection Kits are a series of products for fluorescent double-staining of bacteria. By combining different types of fluorescent stain, staining images can be acquired for each index (membrane impairment, respiratory activity, and esterase activity). Bacterial viability is generally assessed by colony formation in the nutrient-agar medium. However, this requires a long culturing time, and it is hard to recognize the growth of viable but nonculturable (VNC) bacteria. However, fluorescent staining does not require bacterial culture and enables viability assessment by rapid and simple protocols.

-Bacstain- Bacterial Viability Detection Kit – DAPI/PI uses bacterial membrane damage as an index. DAPI is a minor groove binder specific to the AT sequence of DNA and permeates into bacteria to stain nucleic acids regardless of membrane damage. PI is a parallel intercalator into the DNA double helix that stains nucleic acid; it passes only through damaged bacterial membranes. Thus, this kit can be used to measure the ratio of membrane-damaged bacteria to total bacteria on the analysis of the fluorescent images from each stain.

Simple Procedure

Double-staining of Staphylococcus aureus (a Gram-positive bacterium)

Quantitative analysis

-Bacstain- Bacterial Viability Detection Kit – DAPI/PI (BS08) and –CTC/DAPI (BS09) were used to evaluate the effect of benzalkonium chloride on S. aureus (Gram-positive bacteria) by fluorescent imaging. The images were quantified by ImageJ and show the correlation with drug concentration.
The results showed that the effects of benzalkonium chloride on the respiratory activity and membrane damage of S. aureus varied greatly depending on the indicator detected.
The evaluation of multiple indicators will contribute to improving the reliability of drug efficacy measurements by providing a multidimensional view of the activity of the bacterium that would be missed by a single indicator.

Related Product Information

ProductSizeProduct Code
-Bacstain- CFDA solution100 assaysBS03
-Bacstain- DAPI solution100 assaysBS04
-Bacstain- AO solution100 assaysBS05
-Bacstain- PI solution100 assaysBS07
-Bacstain- CTC Rapid Staining Kit (for Flow cytometry)100 assaysBS01
-Bacstain- CTC Rapid Staining Kit (for Microscopy)100 assaysBS02

Related Categories
All Products Bacterial Staining Cellular Assays

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