Polyethyleneglycols (PEGs) are widely used for material modifications to improve hydrophilicity of the surface. PEG-coated materials are usually more stable under physiological conditions. Since Carboxy-EG6-undecanethiol has 6 ethylene glycol units, 11 carbon Atoms, and an SH group at the end, it can be used to prepare a highly oriented and hydrophilic SAM on a gold surface suitable for biomaterial-labelings on the surface due to the improved hydrophilicity. The hydrophilic surface can prevent proteins or other biomaterials from non-specific binding. Therefore, the SAM prepared by this reagent will provide a better surface to develop biomaterial sensors or DNA/protein microarrays. To prepare a carboxy-EG6-SAM on a gold surface, hydroxy-EGn-undecanethiols (n=3, 6) are used to dilute the number of carboxyl groups according to the density of the molecules being introduced onto the surface. There are several papers available on labeling proteins such as ovalbumin and cytochrome C.
When it is difficult to take out all the powder from the container,
please add the solvent into a container and dissolve it before its use.
1) C. Pale-Grosdemange, E. S. Simon, K. L. Prime, and G. M. Whitesides, Anal. Chem., 1999, 71, 777-790.
2) M. Kyo, K.Usui-Aoki, H. Koga, Anal. Chem, 2005, 77, 7115-7121.