Carboxylic acid-SAM Formation Reagent is used to prepare carboxylic acid- terminated self-assembled monolayers (SAMs) on gold surfaces as biosensors detected by QCM (Quartz Crystal Microbalance), SPR (Surface Plasmon Resonance), and electrochemical analysis. Coupled with carboxyl group activation method using NHS/WSC, carboxylic acid-SAM can be used as an interface to immobilize proteins or peptides and other ligands on gold surfaces (Fig. 1). In general, carboxylic acid-SAM provides some non-specific binding because of its negative charge. However, surfaces prepared using -carboxylic acid-SAM Formation Reagent presents less non-specific binding than thiose commonly prepared with carboxylic acid-SAM (Fig. 2).
Fig. 1 A biosensor construction by Carboxylic acid-SAM Formation Reagent
Fig. 2 Comparison of non-specific binding between carboxylic acid-SAM prepared with carboxylic acid-SAM Formation Reagent and commonly prepared SAM