SKU: P378 Categories: , ,

-Cellstain- PI Solution


Chemical Name: 3,8-Diamino-5-[3-(diethylmethylammonio)propyl]-6-phenylphenanthridinium diiodide, aqueous solution
CAS: 25535-16-4(PI)

Product: 1.5 mM PI aqueous solution(1 mg PI per 1 ml water)
Appearance: Orange to red solution
MW: 668.39, C27H34I2N4

Storage Condition : -20ºC, protect from light
Shipping Condition : ambient temperature


Mix and match products can be applied.
You must enter coupon code on your order to receive the discount!
・Summer 21-2 (for buying 2)
・Summer 21-3 (for buying 3 and more)
(Can not be combined with any other offer or special pricing products.)

Product Description
Propidium iodide (PI) is an ethidium bromide analog that emits red fluorescence upon intercalation with double-stranded DNA. PI does not permeate viable cell membranes, but passes through disturbed cell membranes and stains the nuclei. PI is often used in combination with a fluorescein compound, such as Calcein-AM or FDA, for simultaneous staining of viable and dead cells. The excitation and emission wavelengths of PI-DNA complex are 535 nm and 615 nm, respectively.

Chemical Structure

Staining Procedure
1.Prepare 10-50 μM PI solution with PBS or an appropriate buffer.a)
2.Add PI solution with 1/10 of the volume of cell culture medium to the cell culture.b)
3.Incubate the cell at 37oC for 10-20 min.
4.Wash cells twice with PBS or an appropriate buffer.
5.Observe the cells under a fluorescence microscope with 535 nm excitation and 615 nm emission filters.

a) Since PI may be carcinogenic, extreme care is necessary during handling.
b) Or you may replace the culture medium with 1/10 concentration of PI buffer solution.

1) I. W. Taylor, et al., An Evaluation of DNA Fluochromes, Staining Techniques, and Analysis for Flow Cytometry. I. Unperturebed Cellpopulations. J Histochem Cytochem. 1980;28:1224-1232.
2) W. M. J. Vuist, et al., Potentiation by Interleukin 2 of Burkitt’s Lymphoma Therapy with Anti-Pan B (Anti-CD19) Monoclonal Antibodies in a Mouse Xenotransplantation Model. Cancer Res. 1989;49:3783-3788.
3) A. K. El-Naggar, et al., Single- and Double-stranded RNA Measurements by Flow Cytometry in Solid Neoplasms. Cytometry. 1991;12:330-335.
4) C. Souchier, et al., Methods for Cell Proliferation Analysis by Fluorescent Image Cytometry. Cytometry. 1995;20:203-209.
5) T. Irino, et al., Establishment of Real-Time Polymerase Chain Reaction Method for Quantitative Analysis of Asparagine Synthetase Expression. J Mol Diagn. 2004;6:217-224.

Staining Data

Fig. 2 Cell staining with PI

Related Categories
Kits for Life Science Research Cell Staining

Related Products

COVID-19 Announcement

Due to rising concerns regarding the COVID-19 (Coronavirus 2019) outbreak, Dojindo Molecular Technologies, Inc will be taking the necessary steps to prioritize public health and safety.

Scroll to Top