SKU: C309 Categories: , ,



Chemical Name: 5- or 6-(N-Succinimidyloxycarbonyl)fluorescein 3′,6′ diacetate
CAS: 150347-59-4

Appearance: white or slightly yellow solid
MW: 557.46, C29H19NO11

Storage Condition: -20ºC
Shipping Condition: ambient temperature


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CFSE is cell-membrane permeable and readily accumulates inside viable cells where it covalently attaches to intracellular proteins (Fig. 1). Hydrolyzed CFSE emits fluorescence and covalently attached fluorescein molecules do not leak from cells. CFSE-labeled cells can be monitored over several weeks in vivo. Therefore, CFSE is utilized for detection of viable cell as well as for the long-term observation of cell activities by fluorescent microscopy. The excitation and emission wavelengths of CFSE-labeled cells are 500 nm and 520 nm, respectively. CFSE-stained cells are shown in Fig. 2.

Fig. 1 Cell staining mechanism

Staining Procedure
1. Prepare 1 mM CFSE solution with DMSO. Dilute it to prepare 10-50 μM CFSE solution with PBS or an appropriate buffer.
2. Add CFSE solution with 1/10 of the volume of cell culture medium to the cell culture.
3. Incubate the cell at 37ºC for 15 to 30 min.
4. Wash cells twice with PBS or an appropriate buffer.
5. Observe the cells under a fluorescence microscope with 490 nm excitation and 530 nm emission filters.

When it is difficult to take out all the powder from the container,
please add the solvent into a container and dissolve it before its use.

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Fig. 2 Cell staining with CFSE
Cell type: HeLa

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