Chemical Name: Diphenyl-1-pyrenylphosphine
CAS: 110954-36-4

Appearance: slightly yellow powder
Purity: ≥97% (HPLC)
MW: 386.42, C28H19P

Storage Condition: ambient temperature, protect from light
Shipping Condition: ambient temperature

Product Description
DPPP is a non-fluorescent triphenylphosphine compound. It reacts with hydroperoxide to generate DPPP oxide that emits fluorescence at 352 nm excitation and 380 nm emission wavelengths (Fig. 1). Post-column HPLC method is used to determine phospholipid peroxide in sample solutions.

Fig 1. DPPP reaction with lipid peroxidase

When it is difficult to take out all the powder from the container,
please add the solvent into a container and dissolve it before its use.

1) K. Akasaka, et al., Study on Aromatic Phosphines for Novel Fluorometry of Hydroperoxides(II) – the Determination of Lipid Hydroperoxides with Diphenyl-1-Pyrenylphosphine-. Anal Lett. 1987;20:797-807.
2) K. Akasaka, et al., An Aromatic Phosphine Reagent for the HPLC-fluorescence Determination of Hydroperoxides -Determination of Phosphatidylcholine Hydroperoxides in Human Plasma. Anal Lett. 1988;21:965-975.
3) K. Akasaka, et al., A Simple Fluorometry of Hydroperoxides in Oils and Foods. Biosci Biotech Biochem. 1992;56:605-607.
4) K. Akasaka, et al., High-performance Liquid Chromatography and Post-Column Derivatization with Diphenyl-1-Pyrenylphosphine for Fluorimetric Determination of Triacylglycerol Hydroperoxides. J Chromatogr. 1992;596:197-202.
5) K. Akasaka, et al., Simultaneous Determination of Hydroperoxides of Phosphatidylcholine, Cholesterol Esters and Triacylglycerols by Column-Switching High-Performance Liquid Chromatography with a Post-column Detection system. J Chromatogr. 1993;622:153-159.
6) K. Akasaka, et al., Normal-phase High-performance Liquid Chromatograohy with a Fluorimetric Postcolumn Detection System for Lipid Hydroperoxides. J Chromatogr A. 1993;628:31-35.
7) Y. Okimoto, et al., A Novel Fluoresceint Probe Diphenyl-pyrenylphosphine to Follow Lipid Peroxidation in Cell Membranes. FEBS Lett. 2000;474:137-140

Related Categories
Oxidative Stress Array


Related Products

COVID-19 Announcement

Due to rising concerns regarding the COVID-19 (Coronavirus 2019) outbreak, Dojindo Molecular Technologies, Inc will be taking the necessary steps to prioritize public health and safety.

Scroll to Top