DTPA anhydride is utilized to add chelate functions to molecules or surfaces with amine groups. After adding chelate functions, metal ions such as radioisotopes can be introduced to the molecule. According to Dr. Hnatowich, IgG may be labeled with radioactive indium using DTPA-anhydride. DTPA is first linked to IgG through a covalent bond, then In(III) is tightly chelated to DTPA. Such radioactive antibodies are known to bind with antigen in vitro and in vivo.
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