ICG Labeling Kit – NH2


– Suitable wavelength for In Vivo imaging
– Quick and Easy Labeling to Antibodies : 1.5hr
– High Recovery Rate : more than 90%

Contents of the Kit:

Storage: 0-5ºC
Shipping Condition: ambient temperature


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DescriptionReferencesDataQ & AManualS.D.S

Product Description
ICG Labeling Kit – NH2 is used primarily for the preparation of ICG (Iindocyanin green)-labeled antibody for near-infrared fluorescence imaging. ICG offers two remarkable properties:

1) ICG has a strong near-infrared fluorescence even after a few days under physiological conditions. The excitation and emission wavelength of the ICG-labeled proteins are 774 nm and 805 nm, respectively.
2) ICG has been used in clinical fields such as a hepatic deficiency testing. Therefore, ICG and ICG conjugates are materials suitable for in vivo imaging.

This kit contains all required compornents required for labeling, including storage buffer for conjugates. The labeling process is simple:. Add NH2-reactive ICG to protein solution on a filter membrane, and incubate at 37ºC, for 10 minutes. A filtration tube can remove excess ICG molecules.

Fig. 1 Fluorescein Labeling Process to IgG

♦ If the IgG solution contains other proteins with molecular weight greater than 10,000, such as serum albumin or gelatin, purify the IgG solution before labeling fluorescein with this kit. Commercially available antibody may contain BSA or gelatin as a stabilizer. Dojindo offers IgG Purification Kit-A (AP01-10) and IgG Purification Kit-G (AP02-10) for the purification of the IgG solution

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Fig. 3 Fluorescent Property of ICG Dye

Can I use this kit for F(ab')2?

Yes, please follow the labeling protocol for IgG. The recovery of the conjugate should be over 80%.

Can I use this kit for other proteins or peptides?

Yes, if the molecular weight is greater than 50,000.

Can I use this kit to label oligopeptides or oligonucleotides?

No. Oligonucleotides and oligopeptides may be too small to retain on the membrane filter of Filtration Tube.

What is the minimum amount of IgG that can be labeled with LK31-10?

The minimum amount is 50 μg. There is no significant difference in sensitivity and background between 50 μg and 200 μg of IgG. Though 10 μg IgG can still be labeled using this kit, the background will be higher.

How many ICG molecules are introduced to protein?

The number of conjugated ICG depends on the protein. In the case of rabbit IgG, one molecule of ICG conjugate to each protein molecule.

Do I have to use a Filtration tube prior to labeling the protein?

If the protein solution does not contain small molecules with an amino group and the concentration of the protein is 10 mg per ml, or about 70 μM, there is no need to use the Filtration tube. Mix 10 μl of the sample solution with 90 μl of Reaction buffer and add 8 μl NH2-reactive ICG (prepared at step 3) to the mixture, and follow the protocol starting at step 4.

Do I have to use Storage buffer included with the kit?

No, you don’t have to use Storage buffer from the kit. You can choose any kind of buffer appropriate for your experiment.

My sample contains small insoluble material. What should I do?

Spin the sample and use the supernatant for labeling.

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