Isothiocyanobenzyl-NTA is utilized to modify the surfaces on which amine groups are attached. Through the NTA moiety attached on the surface, genetically expressed protein, bearing a hexahistidine extension at its terminus, can be immobilized via Ni (II) (His-Tag method). Using this technique, Dr. Noji and co-workers were able to directly abserve the rotation of F1-ATPase with a fluorescence microscope.
When it is difficult to take out all the powder from the container,
please add the solvent into a container and dissolve it before its use.