The kit is used to detect lactate in cell culture medium by measuring the absorbance of WST formazan produced according to quantities of lactate. The kit includes a “Lactate Standard” which can be used to quantify the concentration of lactate found within samples by creating standard curve.
Simple Assay Procedure
Dojindo’s stock solution is stable for 4 months under refrigerator. Your assay can be done anytime without the need freeze the product and wait for defrosting.
Standard curve can be prepared by using Lactate Standard included in the kit. The concentration of lactate can be measured. The concentration of lactate can be evaluated by diluting the samples if the concentration is over 1 mmol/l.
|S. Hori, Y. Hiramuki, D. Nishimura, F. Sato and A. S. Fujisawa, “PDH-mediated metabolic flow is critical for skeletal muscle stem cell differentiation and myotube formation during regeneration in mice.”, FASEB J. ., 2019, 33(7), 8094.|
|Y. Nonomiya, K. Noguchi, K. Katayama and Y. Sugimoto, “Novel pharmacological effects of poly (ADP-ribose) polymerase inhibitor rucaparib on the lactate dehydrogenase pathway.”, Biochem. Biophys. Res. Commun., 2019, 510(4), 510.|
|K. Ohshima, S. Nojima, S. Tahara, M. Kurashige, K. Kawasaki, Y. Hori, M. Taniguchi, Y. Umakoshi, D. Okuzaki, N. Wada, J. Ikeda, E. Fukusaki and E. Morii, “Serine racemase enhances growth of colorectal cancer by producing pyruvate from serine”, Nat Metab, 2020, 2(1), 81.|
(Blood cells, lymphocytes)
|Y. akashima, A. Hayano and B. Yamanaka, “Metabolome analysis reveals excessive glycolysis via PI3K/AKT/mTOR and RAS/MAPK signaling in methotrexate-resistant primary CNS lymphoma-derived cells.”, Cancer Sci.., 2020, 111(4), 1241-1253.|
|H. Osumi, H. Horiguchi, T. Kadomatsu, K. Tashiro, J. Morinaga, T. Takahashi, K. Ikeda, T. Ito, M. Suzuki, M. Endo and Y. Oike, “Tumor Cell-Derived Angiopoietin-Like Protein 2 Establishes a Preference for Glycolytic Metabolism in Lung Cancer Cells”, Nat Metab, 2020, 2(1), 81|
|T. Matsuo, Y. Konya, E. Hirayama and Y. Sadzuka, “2-Deoxy-D-glucose enhances the anti-cancer effects of idarubicin on idarubicin-resistant P388 leukemia cells”, Oncol Lett , 2020, 20(1), 962-966|
|M. Hashimoto, S. Kimura, C. Kanno, Y. Yanagawa, T. Watanabe, J. Okabe, E. Takahashi, M. Nagano and H. Kitamura, “Macrophage ubiquitin specific protease 2 contributes to motility, hyperactivation, capacitation, and in vitro fertilization activity of mouse sperm”, Cellular and Molecular Life Sciences, 2020, doi: 10.1007/s00018-020-03683-9|