SKU: L261 Categories: ,

LysoPrime Green – High Specificity and pH Resistance

$120.00$250.00

Release Date: 10/29/2021

Content: 10 μL x 1; 10 μL x 3

∼ Features ∼
– High selectivity for lysosomes
– High retention even in lysosomes that had pH neutralized after staining
– Long-term visualization of lysosomes for live cell imaging

The general number of usable assays per 10 μL:
・35 mm dish x 10
・96 well plate x 2

Storage Condition: Store at -80oC
Shipping Condition: with blue ice


*This offer is for the trial size kit of LysoPrime Green (L261-10).

LDs/Mitochondria/Senescence Map Selection Guide
Clear
DescriptionDataManualS.D.S

LysoPrime Green – High Specificity and pH Resistance will be released on October 29th, 2021.

A special discount (50% off) will be offered for a limited time only before release!
To obtain the promotion code, please answer the following short survey!
*This offer is for the trial size of LysoPrime Green (L261-10).

>> Survey Link
(First time US users only)


Detection Principle

Lysosomes are involved in a multitude of cellular processes, and their dysfunction is associated with various diseases. Understanding lysosome-related physiology requires lysosome-specific probes that can track the biological processes of lysosomes within cells. Compared to other subcellular components, lysosomes are the most acidic organelles. Based on this feature, most lysosomal probes have been designed to target the acidic environment within lysosomes.

However, the current strategies for monitoring lysosome activities exhibit several limitations. As consequence of being designed simply to target acidic environments, most lysosomal probes have poor specificity towards the lysosome and stain many non-lysosomal acidic compartments. Moreover, existing lysosome probes are highly reliant on the lysosomal pH value; their emission intensity is unstable and varies significantly due to differences in acidity among lysosomes within a cell.

We have developed a novel fluorescent probe, LysoPrime Green, in order to track the lysosome while avoiding these aforementioned restrictions. Compared to existing lysosome probes, LysoPrime Green shows better selectivity towards lysosomes, and its fluorescence is not affected by variations in lysosome acidity that can occur after staining. In addition, the high-retentivity of LysoPrime Green enables long-term imaging experiments.


LysoPrime Green’s High Selectivity Towards Lysosomes

LysoPrime Green co-localized with LAMP-1 to a higher degree than existing reagent.

 

LysoPrime Green’s Lysosomal pH Independence

Control: Normal condition, Bafilomycin A1: Inhibition of lysosomal acidification

LysoPrime Green retained lysosome staining even in cells that had lysosomal pH neutralized by Baf A1 treatment.

 

Observation of the lysosomal pH change in HeLa cells

Control: Normal condition, Bafilomycin A1: Inhibition of lysosomal acidification
LysoPrime Green filter sets: 488 nm (Ex), 500 – 570 nm (Em)
Reagent LTR (Company T) filter sets: 548 nm (Ex), 550 – 650 nm (Em)

<Microplate reader>

 


Fluorescence Properties

λex : 456 nm
λem : 540 nm

<Recommended filter settings>
Ex : 400 – 490 nm, Em : 500 – 600 nm

Co-Staining with Autophagy Dye and LysoPrime Green under Autophagy-Inducing Conditions

LysoPrime Green retained Lysosome staining after 2 h of starvation, while Reagent LTG (Company T) lost lysosome localization.

*DAPRed (D677): Autophagy detection dye designed for monitoring the autophagosome.

 

Co-Staining with Mitophagy Dye and LysoPrime Green under Mitophagy-Inducing Conditions

LysoPrime Green retained lysosome staining after 24 h FCCP treatment, while Reagent LTG (Company T) lost lysosome localization.

* Mtphagy Dye (MD01): Mitophagy Detection dye designed for monitoring mitophagy.

 

Exosome localization in intracellular organelles

LysoPrime Green retained lysosome staining after 240 min, time-dependent exosome localization in lysosome was observed.

*ExoSparkler Deep Red (EX03) :ExoSparkler designed for exosome membrane labeling.

Related Categories

Intracellular Fluorescent Probes
Shipping Type

Blue Ice ($47.00)

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