It becomes obvious that there are a lots of molecules containing sulfane sulfurs such as persulfides and polysulfides in living organism. These molecular species are involved in production, storage and release of hydrogen sulfide, which is recognized as an important physiological mediator. Furthermore, recent studies reveal that persulfides and polysulfides may control intracellular signal transduction through s-sulfhydration of proteins.
SSP4 (Sulfane Sulfur Probe 4) is a novel fluorescent probe to detect sulfane sulfurs selectively. SSP4 itself is non-fluorescent, but it emits strong green fluorescence when it reacts with sulfane sulfurs. Thus, SSP4 enables high sensitive fluorescence detection and imaging of sulfane sulfurs.
Fig. 1 Chemical Reaction with sulfane sulfer
Fig. 2 Fluorescence spectrum (Em: 482nm/Ex: 515nm)
Chemical species containing sulfane sulfurs
Fig. 3 The formation of sulfane sulfer in vivo changes due to reduction/oxidation and translation
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Left: Control cells
Right: Treatment of 100 μmol/l Na2S3
Cell: CHO cell
Exposure time: 1,000 msc