BS3

09 Cross-Linking Reagents

BS3

BS3

Cross-Linker

  • Product code
    B574　 BS3
  • CAS No.
    82436-77-9(free acid)
  • Chemical name
    Bis(sulfosuccinimidyl)suberate, disodium salt
  • MW
    C16H18N2Na2O14S2=572.43
Unit size Price Item Code
50 mg ＄235.00 B574-10
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50 mg
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Product Description:

BS3 is a cross-linking reagent reacting the amino groups with each other. Since BS3 has at both ends of the molecule N-hydroxysuccinimide active ester to react selectively with the amino group, it can be conjugated a carrier protein to simple hapten or preparation labeled enzyme. BS3 takes a chemical structure having analkyl chain to the linker moiety. Meanwhile, DTSSP and DSP is capable of cleavage of the disulfide-introduced linker site that is easily reduced by the reducing agent. Further, since the active ester group having a sulfonic acid group is introduced to DTSSP and BS3, it may be carried out labeling reaction without using an organic solvent such as DMSO or DMF.

Technical info

Product Name Code Length (Å)
BS3 B574 8.9
DTSSP D630 8.5

References

Open References

1) J. V. Staros, "N-Hydroxysulfosuccinimide Active Ester: Bis(N-hydroxysulfosuccinimide) Ester of Two Dicarboxylic Acids Are Hydrophilic, Membrane-impermeant, Protein Cross-Linkers", Biochemistry, 1982, 21, 3950.
2) J. M. Salhany, R. L. Sloan and K. A. Cordes, "In Situ Cross-linking of Human Erythrocyte Band 3 by Bis8sulfosuccinimidyl)suberate", J. Biol. Chem., 1990, 265(29), 17688.
3) J. E. Gestwicki, C. W. Cairo, L. E. Strong, K. A. Oetjen and L. L. Kiessling, "Influencing Receptor-Ligand Binding Mechanisms with Multivalent Ligand Architecture", J. Am. Chem. Soc., 2002, 124, 14922.
4) S. S. Mark, N. Sandhyarani, C. Zhu, C. Campagnolo and C. A. Batt, "Dendrimer-functionalized Self-assembled Monolayers as a Surface Plasmon Resonance Sensor Surface", Langmuir, 2004, 20, 6808.

Handling and storage condition

Specification
Appearance： White to almost white powder
Purity (HPLC)： ≧93.0 %
Solubility in water： To pass test (clear, colorless)
Handling and storage condition
0-5°C, Protect from moisture
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