09 Cross-Linking Reagents

Isothiocyanobenzyl-NTA

Cross-Linking Reagents

Chelate Labeling Reagent

Product code

I279　 Isothiocyanobenzyl-NTA
Chemical name

N-[5-(4-IsothiOcyanatobenzyl)amido-1-carboxypentyl]iminodiacetic acid
MW

C₁₉H₂₃N₃O₇S=437.47

Unit size	Price	Item Code
10 mg	＄265.00	I279-10

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10 mg

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Product Description

Isothiocyanobenzyl-NTA is utilized to modify the surfaces on which amine groups are attached. Through the NTA moiety attached on the surface, genetically expressed protein, bearing a hexahistidine extension at its terminus, can be immobilized via Ni (II) (His-Tag method). Using this technique, Dr. Noji and co-workers were able to directly abserve the rotation of F1-ATPase with a fluorescence microscope.