1-Methoxy PMS

10 Redox Dyes

1-Methoxy PMS

1-Methoxy PMS

Electron Mediator

  • Product code
    M003　 1-Methoxy PMS
  • CAS No.
    65162-13-2
  • Chemical name
    1-Methoxy-5-methylphenazinium methylsulfate
  • MW
    C15H16N2O5S=336.36
Unit size Price Item Code
100 mg ＄108.00 M003-10
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100 mg
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Reaction Scheme

Product Description

1-Methoxy PMS is easily dissolved by water and alcohol. Its redox potential is +63 mV. Though phenazinium methylsulfate (PMS) is commonly used as an electron carrier for NADH-tetrazolium, the stability of PMS is very poor. However, 1-Methoxy PMS solution can be stored at room temperature for over 3 months without protection from light. An electron of NAD(P)H is transfered by 1-Methoxy PMS to tetrazolium salt (Reaction Scheme). Therefore, it is a useful reagent for NAD(P)H-tetrazolium-based assay systems.

References

Open References

1) R. Hisada and T. Yagi, "1-methoxy-5-methylphenazinium Methyl Sulfate", J. Biochem., 1977, 82, 1469. 
2) 八木達彦, "1-メトキシ PMS", Dojin News, 1979, 14, 1. 
3) S. Nakamura, K. Arimura, K. Ogawa and T. Yagi, "Use of 1-methoxy-5-methylphenazinium Methyl Sulfate(1-methoxypms) in the Assay of Some Enzymes of Diagnosticimportance", Clin. Chim. Acta,, 1980, 101, 321.
4) R. Hisada, W. Shinkai and T. Yagi, "Photochemical Stabilities and Biochemical Reactivities of Some Derivatives of 5-methylphenazinium methyl Sulfate(Phenazine Methosulfate)", J. Appl. Biochem., 1981, 3, 535. 
5) C. J. von Noorden and J. Tas, "The Role of Exogenous Electron Carriers in NAD(P)-dependent Dehydrogenase Cytochemistry Studied in vitro and with a Model System of Polyacrylamide Films", J. Histochem. Cytochem., 1982, 30, 12. 
6) H. Tsuge, Y. Kuroda, A. Iwamoto and K. Ohashi, "Partial Purification and Property of Pyridoxine (Pyridoxamine)-5'-phosphate Oxidase Isozymes from Wheat Seedlings", Arch. Biochem. Biophys., 1982, 217, 479. 
7) M. Rabinovitch, J. P. Dedet, A. Ryter, R. Robineaux, G. Topper and E. Brunet, "Destruction of Leishmania Mexicana Amazonensis Amastigotes within Macrophages in Culture by Phenazine Methosulfate and Other Electron Carriers", J. Exp. Med., 1982, 155, 415. 

Handling and storage condition

Specification
Appearance： Dark red to reddish purple powder
Purity (Absorbance)： ≧ 95.0 %
Solubility in water： To pass test (clear, pink)
Molar absorptivity： ≧ 2,700 (around 505 nm)
m.p.： ≧ 170oC (dec.)
IR spectrum： Authentic
Handling and storage condition
Ambient temperature
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