Metabolism Promotion 2022

Why is cancer cell metabolism in the spotlight now?

Tumor development and progression require metabolic reprogramming of cancer cells. Cancer cells autonomously alter fluxes through various metabolic pathways to meet increased bioenergetic and biosynthetic demands as well as to reduce oxidative stress necessary for proliferation and survival*. Elucidating the triggers of these metabolic changes in cancer cells will lead to new possibilities for cancer therapy.

*Inmaculada Martinez-Reyes & Navdeep S. Chandel, Cancer metabolism: looking forward, Nature Reviews Cancer, 21, 669-680 (2021)


Promotional Products by Category

Category Product Unit Price

Cellular
Metabolism

ATP Assay Kit-Luminescence 50 / 200 tests $250.00 / $450.00
Glucose Assay Kit-WST (Colorimetric) 50 / 200 tests $185.00 / $400.00
Lactate Assay Kit-WST (Colorimetric) 50 / 200 tests $297.00 / $714.00
Glutamine Assay Kit-WST (Colorimetric) 100 tests $564.00
Glutamate Assay Kit-WST (Colorimetric) 100 tests $513.00
α-Ketoglutarate Assay Kit-Fluorometric 100 tests $660.00
NAD/NADH Assay Kit-WST (Colorimetric) 100 tests $568.00
NADP/NADPH Assay Kit-WST (Colorimetric) 100 tests $568.00
Glycolysis/OXPHOS Assay Kit NEW 50 tests $480.00

Nutrient
Uptake

Glucose Uptake Assay Kit-Green NEW 50 / 100 tests $150.00 / $380.00
Glucose Uptake Assay Kit-Blue / Red NEW 100 tests $400
Amino Acid Uptake Assay Kit NEW 20 / 100 tests $160.00 / $450.00
Cystine Uptake Assay Kit NEW 20 / 100 tests $180.00 / $500.00


Combination Assay with Metabolism Kits & Cell Counting Kit-8

Energy metabolism shifts by 2-Deoxy-D-glucose (2-DG) and oligomycin in HeLa cells

We inhibited glycolytic and mitochondrial oxidative phosphorylation in HeLa cells, respectively, and then evaluated metabolic shift and cell viability.

Step 1. Confirm whether ATP production changes from glycolysis to mitochondrial oxidative phosphorylation
Step 2. Investigate changes in extracellular lactate levels as a measure of glycolytic activity
Step 3. Measure cell viability under the inhibition condition of each glycolysis and mitochondrial oxidative phosphorylation.

In conclusion,

1. Inhibition of the glycolytic pathway and oxidative phosphorylation in mitochondria, respectively, causes a metabolic pathway switch in HeLa cells.
2. Inhibition of both pathways, rather than inhibition alone, showed more effective anticancer activity.

 Step 1.

Step 2.  

Related products

ATP Assay Kit-Luminescence
Lactate Assay Kit-WST (Colorimetric)
Glycolysis/OXPHOS Assay Kit (ATP Assay Kit, Lactate Assay Kit, 2-DG, and oligomycin all-in-one kit)
Cell Counting Kit-8 Free in this promotion!

Other recommended combinations involving our metabolism kits


Learn more

The advantages of each metabolism kit

Cell Counting Kit-8: Best choice for accurate data

For first-time researchers of cell proliferation/cytotoxicity

 

Product Classification

Product Classification