Cellular Senescence and Neurodegeneration: the Importance of Mitochondria-Lysosome Cross-talk DOJINDO LABORATORIES

Mitochondria and lysosomes serve as fundamental signaling platforms within the cell, regulating many important processes such as cellular senescence. Disruption of the crosstalk between mitochondria and lysosome may serve as a trigger that converts normal aging into neurodegeneration*. From this perspective, understanding the connections between these organelles and the cellular responses they elicit may reveal so-far-unexplored targets that can be of therapeutic value.

*Deus CM. et al., Mitochondria-Lysosome Crosstalk: From Physiology to Neurodegeneration, Trends. Mol. Med., 2020, 26, 1.

Indicators of Senescence	Reagent	Related Reference
SA-βGal (⬆)	Cellular Senescence Detection Kit – SPiDER-ßGal	Saez- Atienzar S. et al., Cellular senescence and Alzheimer disease: the egg and the chicken scenario, Nat. Rev. Neurosci., 2020, 21, 433–444
Mitochondrial ROS (⬆)	MitoBright ROS Deep Red – Mitochondrial Superoxide Detection	Willey CD. et al., Mitochondrial Dysfunction Induces Senescence with a Distinct Secretory Phenotype, Cell Metab., 2016, 23, 303–314
Mitophagy (⬇)	Mitophagy Detection Kit	Korolchuk VI. et al., Mitochondria in Cell Senescence: Is Mitophagy the Weakest Link?, eBioMedicine, 2017, 21, 7-13.
Mitochondrial Membrane Potential (⬇)	JC-1 MitoMP Detection Kit	Li Y. et al., A Newly Synthesized Rhamnoside Derivative Alleviates Alzheimer’s Amyloid- β-Induced Oxidative Stress, Mitochondrial Dysfunction, and Cell Senescence through Upregulating SIRT3, Hindawi., 2020, 7698560
Lysosomal Mass (⬆)	LysoPrime Green – High Specificity and pH Resistance	Kurz DJ. et al., Senescence-associated b-galactosidase reflects an increase in lysosomal mass during replicative ageing of human endothelial cells, J. Cell Sci.., 2000, 113, 3613–3622
Cell Size (⬆)	PlasMem Bright Green	Lengefeld J. et al., Cell size is a determinant of stem cell potential during aging, Sci. Adv., 2021, 7, eabk0271
Glucose Uptake (⬆)	Glucose Uptake Assay Kit-Green	Yao GD. et al., Blocking the utilization of glucose induces the switch from senescence to apoptosis in pseudolaric acid B-treated human lung cancer cells in vitro, Acta. Pharmacol. Sin., 2017, 1401-1411

Indicators of Senescence

Reagent

Related Reference

SA-βGal (⬆)

Cellular Senescence Detection Kit – SPiDER-ßGal

Saez- Atienzar S. et al., Cellular senescence and Alzheimer disease: the egg and the chicken scenario, Nat. Rev. Neurosci., 2020, 21, 433–444

Mitochondrial ROS (⬆)

MitoBright ROS Deep Red – Mitochondrial Superoxide Detection

Willey CD. et al., Mitochondrial Dysfunction Induces Senescence with a Distinct Secretory Phenotype, Cell Metab., 2016, 23, 303–314

Mitophagy (⬇)

Mitophagy Detection Kit

Korolchuk VI. et al., Mitochondria in Cell Senescence: Is Mitophagy the Weakest Link?, eBioMedicine, 2017, 21, 7-13.