01 Cell Proliferation / Cell Cytotoxicity Assay

Viability/Cytotoxicity Multiplex Assay Kit

Viability/Cytotoxicity Multiplex Assay Kit

Cell Proliferation / Cytotoxicity Assay Kit

Set of Cell Counting Kit-8 and Cytotoxicity LDH Assay Kit-WST (500 tests each)
  • Product code
    CK17  Viability/Cytotoxicity Multiplex Assay Kit
Unit size Price Item Code
500 tests $341.00 CK17-10

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500 tests
Quantity may be adjusted within the cart.
Component
500 tests Cell Counting Kit-8
[500 tests]
・WST-8, 1-Methoxy PMS

Cytotoxicity LDH Assay Kit-WST
[500 tests]
・Dye Mixture      
・Assay Buffer
・Lysis Buffer
・Stop Solution
5ml x1





x1
55 ml x1
5.5 ml x1
27.5 ml x1

Product Description

In order to confirm cytotoxicity, evaluation using only live cells or only dead cells as indicators may not be sufficiently reliable, and therefore, there are an increasing number of cases where experiments are supported by evaluating multiple indicators based on different measurement principles.

This kit is a set of two different indicators, Cell Counting Kit-8 (CCK-8) and Cytotoxicity LDH Assay Kit-WST (LDH Assay Kit). An assay manual is available to evaluate these two assays using the same sample.

Technical info

When confirming cytotoxicity, evaluation using only live cells or only dead cells as indicators may not be sufficiently reliable, and therefore, more and more experiments are being supported by evaluation using multiple indicators based on different measurement principles.

 

Experimental Example

Cell Counting Kit-8 and Cytotoxicity LDH Assay Kit-WST measure dehydrogenase activity and released amount of LDH as reflective of living cells and dead cells, respectively. According to the results with anticancer drug, the  number of living cells was reduced, and the number of dead cells increased as the concentration of Mitomycin C increased. Interestingly, a low concentration of Mitomycin C (green frame) inhibits cell proliferation earlier than inducing cell death unlike a high concentration (red frame). 

 

Measuring Principle

LDH Assay Kit: The activity of LDH leaked out of cells due to cell membrane damage can be confirmed as an indicator of dead cells.
Cell Counting Kit-8: Metabolic activity in living cells can be confirmed using NADH as an indicator.

The same cell sample can be applied

This kit includes CCK-8 for measuring live cells and LDH Assay Kit for measuring dead cells, both of which can be evaluated by plate assay at the same absorbance wavelength. The same cell sample can be used to evaluate both live and dead cells at the same time, saving time and labor.

References evaluating cytotoxicity using CCK-8 and the LDHAssay Kit.

Cell Type Smaple Title of reference Reference
C3H10T1/2 Pirfenidone XL413, a cell division cycle 7 kinase inhibitor enhanced the anti-fibrotic effect of pirfenidone on TGF-s1-stimulated C3H10T1/2 cells via Smad2/4) S. Jin, et. al, Exp Cell Res., 2015339(2), 289-99
Human liver cell line L02 S17092
(POP inhibitor)
Prolyl Oligopeptidase Inhibition Attenuates Steatosis in the L02 Human Liver Cell Line Zhou D, et al., PLOS ONE201611(10)
RAW264.7 FAC
(Ferric ammonium citrate)
IFN-gamma
Iron Reduces M1 Macrophage Polarization in RAW264.7 Macrophages Associated with Inhibition of STAT1 Zhen-Shun Gan, et. al, Mediators of Inflammation2017
Mouse embryonic
Fibroblast (MEF)
Methylmercury
(MeHg)
Atg5-dependent autophagy plays a protective role against methylmercury-induced cytotoxicity Takanezawa Yasukazu, et. alToxicity Letters2016, 262, 135-141

References

Open References

1) R. Watanabe, T. Kurose, Y. Morishige and K. Fujimori, "Protective Effects of Fisetin Against 6-OHDA-Induced Apoptosis by Activation of PI3K-Akt Signaling in Human Neuroblastoma SH-SY5Y Cells."Neurochem. Res.., 2018, 43, (2), 488-499.

Handling and storage condition

Handling and storage condition
0-5°C,Protect from light
Danger / harmful
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